A Universal Aptamer for SARS-CoV-2 Detection

We’re excited to share our latest work on SARS-CoV-2 where we expand on our previous publications and describe an aptamer class that binds all major variants of concern with high affinity. This is an important tool for the development of aptamer based COVID detection technologies that can be specific, sensitive and robust for the detection of current and possibly future variants. Read more about our work here. -Jim

Zhang Z, Li J, Gu J, Amini R, Stacey HD, Ang JC, White D, Filipe CDM, Mossman K, Miller MS, Salena BJ, Yamamura D, Sen P, Soleymani L, Brennan JD, Li Y. A Universal DNA Aptamer that Recognizes Spike Proteins of Diverse SARS-CoV-2 Variants of Concern. Chemistry. 2022 Feb 26:e202200524. doi: 10.1002/chem.202200524. Epub ahead of print. PMID: 35218097.

Aptamers from random sequence space: Accomplishments, gaps and future considerations

Aptamers are nucleic acids that are capable of recognizing target molecules. Over the past 30 years, thousands of aptamers have been discovered by scientists via in vitro selection technique. This review examined the range of targets as well as the affinity and specificity of ~1000 generated aptamers. By comparing the synthetic aptamers with natural riboswitches, protein-based recognition elements, a gap between synthetic aptamers and natural recognition elements was revealed.  A series of ideas for developing better aptamers were proposed to solve the real world problems. We hope these strategies can help aptamers remain high functionality in application environment. Happy reading 😊 – Shuwen Qian

Qian, S.; Chang, D.; He, S.; Li, Y. Aptamers from Random Sequence Space: Accomplishments, Gaps and Future Considerations. Analytica Chimica Acta 2022, 1196, 339511. https://doi.org/10.1016/j.aca.2022.339511

Applying our SARS-CoV-2 Aptamers for Diagnostics

Following up on our recent report of high-affinity DNA aptamers for SARS-CoV-2, we’ve been busy working with our collaborators in the Soleymani Lab and the Biointerfaces Institute to apply them on electrochemical sensors. Read our latest COVID19 paper at Pubmed to learn more about how we achieve high sensitivity detection of SARS-CoV-2 in unprocessed human saliva to pave the way for a rapid and sample diagnostic test.

Facile Synthesis of Pd-Ir Nanocubes for Biosensing

Take a quick look at our recent publication in Frontiers in Chemistry. In this paper, a simple and surfactant-free approach is presented to synthesize Pd-Ir nanocubes with atomic Ir shell thickness in an aqueous solution at room temperature. Biomolecules such as antibodies and nucleic acids have free access to the surface of Pd-Ir nanocubes. Applications of Pd-Ir nanocubes in immunoassays and aptamer-based biosensors are realized, exploiting the excellent peroxidase activity and fluorescence quenching ability of Pd-Ir nanocubes. Read more about our work at Pubmed. – Jiuxing

Li J, Li Y. Facile Synthesis of Pd-Ir Nanocubes for Biosensing. Front Chem. 2021 Nov 24;9:775220. doi: 10.3389/fchem.2021.775220. PMID: 34900937; PMCID: PMC8651546.

DNA Aptamers for SARS-CoV-2

The Li Lab and our collaborators from across the McMaster community have published our first report on our novel aptamers for COVID19! The lab has refocused our efforts over the past year to address pressing COVID19 problems and we’re excited to finally be sharing our work. Read more about our work at Pubmed.

NSERC & L’Oréal-UNESCO Women in Science and IUPAC Young Chemist

Li Lab postdoctoral fellow Erin McConnell has been awarded the NSERC & L’Oréal-UNESCO Women in Science Supplement. Congratulations to Erin for her fantastic work as a scientist and as a role model for young scientists around the world.

Explore the following links to learn more about Erin’s work and the work of the other great researchers recently recognized.

Canadian Awarding ceremony of the L’Oréal-UNESCO For Women in Science fellowships

NSERC and L’Oréal-UNESCO For Women in Science Supplement

L’Oréal-UNESCO For Women in Science 2018: Erin McConnell

IUPAC Young Chemist

https://iupac.org/100/pt-of-chemist/#erin-mcconnell-he

A DNAzyme Feedback Amplification Strategy for Biosensing

Take a look at this recent publication from the Li Lab in Angewandte Chemie International Edition. In this paper, we explore a novel signal amplification strategy termed DNAzyme feedback amplification (DFA). This method takes advantage of rolling circle amplification and RNA-cleaving DNAzymes for biosensing applications, with sensitivity improvements of 3-6 orders of magnitude when compared to conventional methods. -Suraj

Detection of DNA Amplicons of Polymerase Chain Reaction Using Litmus Test

Checkout this recent paper from the Li Lab in Scientific Reports, “Detection of DNA Amplicons of Polymerase Chain Reaction Using Litmus Test.” Here we report on a novel DNA detection method that combines the advantages of the polymerase chain reaction with the simplicity of a litmus test. The diagnostic capabilities of the platform is demonstrated using clinically validated stool samples from C.difficile infected patients. -Suraj

 

 

Funding for Legionella Biosensors Brings Research and Industry Together

Funding was recently announced to advance the Li lab’s research into rapid Legionella diagnostic technologies. Congratulations to our McMaster collaborators Dr. Carlos Filipe and Dr. John Brennan of the Biointerfaces Institute  and industry partners TGWT Clean Technologies Inc., Cytodiagnostics and Mold and Bacterial Consulting Labs. Read more about it on the McMaster News website.